Site-directed mutagenesis using altered beta-lactamase specificity.
نویسندگان
چکیده
Promega's new GeneEditorTM in vitro Site-Directed Mutagenesis System* provides a high efficiency, reliable procedure for the generation and selection of oligonucleotide-directed mutants. The Selection Oligonucleotides provided in this system encode mutations which alter the substrate specificity of beta-lactamase, the gene encoding ampicillin resistance, commonly found on cloning vectors. This altered substrate specificity provides a positive selection for the mutant strand and results in consistently high mutagenesis frequencies using either double-stranded (ds) or single-stranded (ss) DNA templates.
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عنوان ژورنال:
- Methods in molecular biology
دوره 182 شماره
صفحات -
تاریخ انتشار 2002